VOLUME 72 : 2024

Isolation and Identification of Fungal Endophytes Associatedwith Leaves of Rhizophora mucronata Lamk.

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Online Publication Date: April 2024

Jayzon G. Bitacura, James Kennard S. Jacob and Thomas Edison E. dela Cruz

Mangrove-associated fungal endophytes (MFE) produce structurally diverse secondary metabolites with
promising pharmaceutical applications. In our effort to search for these valuable fungi, reported herein is
our isolation and molecular identification of fungal endophytes associated with mature leaves of the host
mangrove Rhizophora mucronata. The isolated MFE were characterized based on their colonial growth on
three culture media – potato dextrose agar (PDA), Czapek Dox agar (CDA), and malt extract agar (MEA),
and these were grouped into 16 morphospecies. Analysis of the ITS genes confirmed the identities of the
isolated mangrove fungal endophytes as belonging to the genera Aspergillus, Cladosporium, Curvularia,
Diaporthe, Fusarium, Nigrospora, Penicillium, Pestalotiopsis, and Schizophyllum. Our study showed the
species richness of fungal endophytes associated with Philippine mangroves.


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