VOLUME 69 : 2021
Utilization of Danio rerio (zebrafish) embryos to compare
the modulatory effects of Abelmoschus esculentus (L.)
(okra), Curcuma longa (L.) (turmeric) and Moringa oleifera
(Lam.) (malunggay) aqueous extracts on the development
and expression of selected gene markers
PAGE 27-44
Kirsten Margarette N. Evidente, Adam Daniel S. Galang, Immanuel Christian V. Fontanilla, Rosheen Angel M. Villaraza, Aila M. Mariano, Mariah Odelle E. Quintano, Dana A. Young, Dana Fay T. Yu, Mariella Cielo S. Cobarrubias, Yrish Shane G. de Guzman, Anna Francine N. Felipe, Denise Marinelle B. Rivera and Marilyn G. Rimando
ARTICLE DOI: https://doi.org/10.53603/actamanil.69.2021.brlg5871
Abelmoschus esculentus (okra), Curcuma longa (turmeric) and Moringa oleifera (malunggay) are common and widely available sources of medicinal phytoconstituents. In this paper, we examined the feasibility of using Danio rerio (zebrafish) embryos in investigating the effect of the aqueous extracts from these three plant sources on the development and expression of genes associated with three metabolic conditions: osteoarthritis, diabetes and auto-immune diseases. Zebrafish embryos at different developmental stages: 48, 72, 96 and 168 hours post fertilization (hpf) were exposed to various concentrations of the aqueous extracts of okra, turmeric or malunggay and the effect on morphology, survival and gene expression was examined. Total RNA from the embryos was isolated and the gene expression was determined by SBYR green quantitative-real time PCR.
FOLLOW US
-
Research Center for the Natural and Applied Sciences
Thomas Aquinas Research Complex Building
University of Santo Tomas España, 1015 Manila, Philippines -
TL: (+63 2) 3406-1611 local 4037
DL: (+63 2) 8731-4031 - actamanilana@ust.edu.ph
© 2023 University of Santo Tomas, Acta Manilana. All rights reserved
Powered by: Communications Bureau