VOLUME 65 : 2017

VOLUME 65 : 2017

ACTA MANILANA publishes research and innovation in the different branches of the natural and applied sciences. It reports significant development in the discipline, and novel applications, unconfined by the traditional coverage of the disciplines.

A glucomannan with alpha-glucosidase inhibitory activity isolated from the leaves of Antidesma microcarpum Elmer (Euphorbiaceae)

Page 11–16

John P. Paulin, Mario A. Tan, Grecebio Jonathan D. Alejandro, Mafel C. Ysrael

The alpha-glucosidase inhibition is seen as a possible approach to controlling post-prandial hyperglycemia in diabetes mellitus. Some species of the genus Antidesma L. are reported to be of use in the treatment of diabetes. In search of indigenous and/or endemic Antidesma species with potential use as adjuvants of antidiabetic agents the extracts of A. curranii, A. fusicarpumA. microcarpum, and A. montanum were screened for alpha-glucosidase inhibitory activity and to isolate the active constituent from the most active extract. The Antidesma extracts were assayed by an in vitro method using yeast alpha-glucosidase. The in vitro alpha-glucosidase inhibitory assay was also used for the bioassay-guided isolation of the active constituent. A. microcarpum extract exhibited the highest glucosidase inhibitory activity with an IC50 of 1.42±0.03 upsilon g/mL. The bioassay-guided isolation of the active constituent in the extract of A. microcarpum afforded a glucomannan having the structure beta-Dmannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->4)-[beta-D-glucopyranosyl-(1->3)]-alpha-Dglucopyranose and with an IC50 alpha-glucosidase inhibitory activity of 0.22 ±0.04 upsilon g/mL which is higher than the inhibitory activity of standard acarbose.

Keywords: Antidesma microcarpum, Euphorbiaceae, alpha-glucosidase inhibitory activity, glucomannan


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