VOLUME 62 : 2014

VOLUME 62 : 2014

ACTA MANILANA publishes research and innovation in the different branches of the natural and applied sciences. It reports significant development in the discipline, and novel applications, unconfined by the traditional coverage of the disciplines.

The first report of a Salmonella enterica serovar Havana phage and its lytic activity at storage temperature of processed chicken

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Ramon Arvin Noriel B. Santos, Mary Anne Claudine S.P. Avena, Rose Elaine J. Gumafelix, Gillian Anthony A. Mamuric, Anna Kristina D. Pastoral, & Donna May D. Papa

Graphical Abstract


Salmonella enterica serovar Havana was isolated from raw, unprocessed chickens. A bacteriophage, designated as Bacteriophage A3CE, capable of lysing S. Havana was isolated from soil. It possesses an icosahedral head, a contractile tail, and linear, double-stranded DNA genome classified under the Family Myoviridae. There was a >90% reduction in the number of host, given that the ratio of phage A3CE to S. Havana was 10 or more (MOI>10), 6 h after the bacteriophage was introduced to the host at room temperature. Phage A3CE and its host were mixed and incubated at both room temperature and 4°C (same concentration for both temperatures). Results show that there is no significant difference between the activity of the isolated phage at room temperature and at 4°C (p>0.05). Thus, Phage A3CE was effective in reducing the number of S. Havana at both temperature conditions making it a promising alternative agent against the said bacterium. This was the first study to document S. Havana phages and was the first recorded Salmonella phage in the Philippines.

Keywords: Salmonellosis, Myoviridae, Multiplicity of Infection, storage temperature,
room temperature


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