ACTA MANILANA

ACTA MANILANA

Phytochemical analysis, antioxidant, and antimutagenic potentials of Greeniopsis sibuyanensis Elmer

Phytochemical analysis, antioxidant, and antimutagenic potentials of Greeniopsis sibuyanensis Elmer

Gina C. Castro, Grecebio Jonathan D. Alejandro, Maria Cristina R. Ramos & Mafel C. Ysrael
Page 25-32

Greeniopsis Merr. is one of the poorly known endemic genera of the Philippine Rubiaceae.
There is currently little published information on the phytochemistry and biological activities
of Greeniopsis species, while important biological activities have been documented for
several species of its sister-genus, Ixora L. This study aimed to screen for the presence of
major chemical constituents and investigate the antioxidant and antimutagenic potentials of
the methanolic leaf extract of Greeniopsis sibuyanensis Elmer. Standard phytochemical
screening methods determined the class of plant constituents. The DPPH (2,2-diphenyl-1-
picryhydrazyl) radical scavenging and the lipid peroxidation inhibition on -carotene linoleate
model system (-CLAMS) assays tested the antioxidant activities of the extract against -
Tocopherol. The SOS-red fluorescent protein bioassay system (SOS-RFP) measured the
anti-mutagenic activity of the extract. Phytochemical screening confirmed the presence of
alkaloids, steroids, terpenoids, flavonoids, saponins, and tannins. Total phenolic content
(TPC) and total flavonoid content (TFC) were found to be 20.06 mg gallic acid equivalents
and 4.29 mg quercetin equivalents per gram crude extract, respectively. The EC50 values in
the DPPH radical scavenging assay was 42.40 g/mL for -Tocopherol, and 650.72 g/mL
for the crude extract. The antioxidant activity based on lipid peroxidation inhibition in the -
CLAMS was found to be 76.84% and 22.20% for -Tocopherol (1000 ppm) and crude
extract (2500 ppm), respectively. The crude extract was non-mutagenic in the SOS-RFP. It
was antimutagenic against mitomycin C-induced damage with an IC50 of 174.84 ppm. The
preliminary data on the secondary metabolites of G. sibuyanensis and the observed antioxidant
and anti-mutagenic properties may lead to further chemical and biological investigation for
its utility to health and wellness.

University of Santo Tomas, Acta Manilana